Glucagon-mediated Ca21 signaling in BHK cells expressing cloned human glucagon receptors
نویسندگان
چکیده
LARS H. HANSEN,1 JESPER GROMADA,2 PIERRE BOUCHELOUCHE,3 TED WHITMORE,4 LAURA JELINEK,4 WAYNE KINDSVOGEL,4 AND ERICA NISHIMURA1 1Department of Molecular Signaling, Hagedorn Research Institute, DK-2820 Gentofte; 2Islet Cell Physiology, Novo Nordisk A/S, DK-2100 Copenhagen; 3Department of Clinical Biochemistry, Roskilde County Hospital, DK-4600 Køge, Denmark; and 4ZymoGenetics Inc., Seattle, Washington 98102
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Glucagon-mediated Ca2+signaling in BHK cells expressing cloned human glucagon receptors.
From video imaging of fura 2-loaded baby hamster kidney (BHK) cells stably expressing the cloned human glucagon receptor, we found the Ca2+ response to glucagon to be specific, dose dependent, synchronous, sensitive to pertussis toxin, and independent of Ca2+ influx. Forskolin did not elicit a Ca2+response, but treatment with a protein kinase A inhibitor, the Rp diastereomer of 8-bromoadenosine...
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